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Related Experiment Videos

The polymerase chain reaction: from functional genomics to high-school practical classes

G R Taylor1, P Robinson

  • 1DNA Laboratory, St James University Hospital, Leeds, UK.

Current Opinion in Biotechnology
|March 21, 1998
PubMed
Summary

Polymerase chain reaction (PCR) is the standard for DNA and RNA analysis, with ongoing advancements in automation and specialized techniques enhancing its utility in molecular biology research and diagnostics.

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Methods in molecular medicine·2011

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Genomics

Background:

  • Polymerase chain reaction (PCR) has been a key in vitro method for DNA cloning for ten years.
  • It is now the standard technique for DNA and RNA analysis in research and diagnostics.

Purpose of the Study:

  • To review recent advancements in PCR technology.
  • To highlight the expanding applications of PCR in molecular biology.

Main Methods:

  • Review of recent developments in PCR technology, including improved thermal cyclers, labeling, and detection methods.
  • Examination of trends towards automation in PCR.
  • Discussion of modified PCR techniques like long-accurate PCR and arrayed oligonucleotides.

Main Results:

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  • PCR is firmly established as the primary method for DNA and RNA analysis.
  • Improvements in robustness, thermal cyclers, and detection have strengthened its position.
  • Increasing automation is a significant trend, though diagnostic kit adoption is limited.
  • New PCR applications are emerging through modifications like long-accurate PCR.

Conclusions:

  • PCR technology continues to evolve, solidifying its role in molecular diagnostics and research.
  • Further automation and specialized PCR variants promise to expand its analytical capabilities.