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Macromolecular matchmaking: advances in two-hybrid and related technologies
1Howard Hughes Medical Institute, University of Washington, Seattle 98195, USA. rfreder@genetics.washington.edu
Current Opinion in Biotechnology
|March 21, 1998
Abstract:
The success of the original yeast two-hybrid system has stimulated the development of a number of 'hybrid technologies' in yeast (and now prokaryotes and mammals) to widen the scope of the protein-protein interactions that can be analyzed, and to enable comparable studies of the interactions of proteins with DNA, RNA or small chemical ligands. In addition, the application of the two-hybrid system to entire genomes is being used to create protein linkage maps which catalog the network of interactions of an organism's complete proteome.