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The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis
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Published on: March 17, 2010

BIAcore for macromolecular interaction

M Fivash1, E M Towler, R J Fisher

  • 1Data Management Services, Inc., Frederick, MD, USA.

Current Opinion in Biotechnology
|March 21, 1998
PubMed
Summary
This summary is machine-generated.

Surface Plasmon Resonance (SPR) spectroscopy, particularly with BIAcore technology, is increasingly popular for studying biospecific interactions. However, quantitative data and binding models in published research often require improvement.

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Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)

Published on: November 29, 2014

Area of Science:

  • Biophysics
  • Analytical Chemistry
  • Biochemistry

Background:

  • Surface Plasmon Resonance (SPR) spectroscopy is a widely used technique for analyzing molecular interactions.
  • BIAcore technology is a prominent platform for SPR measurements.
  • The literature review covers recent advancements and applications in SPR spectroscopy.

Purpose of the Study:

  • To review the literature on Surface Plasmon Resonance (SPR) spectroscopy applications using BIAcore technology.
  • To assess the current state of SPR-based biospecific interaction analysis.
  • To identify trends in the application and reporting of SPR data.

Main Methods:

  • Literature search for studies employing SPR spectroscopy with BIAcore technology.
  • Analysis of nearly two hundred relevant citations.
  • Evaluation of qualitative and quantitative aspects of reported experiments.

Main Results:

  • A significant increase in the use of SPR spectroscopy with BIAcore technology was observed.
  • New applications and modifications of standard SPR techniques have been developed.
  • While qualitative experimental aspects are generally excellent, quantitative data (e.g., kinetic rate constants) and binding models are often lacking or insufficient.

Conclusions:

  • SPR spectroscopy, especially with BIAcore, is a rapidly growing field for biospecific interaction analysis.
  • There is a need for more rigorous quantitative analysis and appropriate binding model selection in published SPR studies.
  • Future research should focus on improving the quantitative reporting of SPR experiments to fully leverage the technique's potential.