Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Large-scale purification of hepatitis B surface antigen

J Vnek, A M Prince

    Journal of Clinical Microbiology
    |June 1, 1976
    PubMed
    Summary

    This study presents a simple two-step method to purify Hepatitis B surface antigen (HBsAg) from plasma. The process achieved significant purification, making HBsAg more accessible for research and diagnostics.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Methodology Used in DNA-Based Prophylactic and Therapeutic Immunization Against Hepatitis B Virus in Chimpanzees.

    Methods in molecular medicine·2011
    Same author

    Development of the International Consortium for Blood Safety (ICBS) HCV panels.

    Eastern Mediterranean health journal = La revue de sante de la Mediterranee orientale = al-Majallah al-sihhiyah li-sharq al-mutawassit·2008
    Same author

    Schistosoma infection inhibits cellular immune responses to core HCV peptides.

    Parasite immunology·2005
    Same author

    Hepatitis C virus replication kinetics in chimpanzees with self-limited and chronic infections.

    Journal of viral hepatitis·2004
    Same author

    Perspectives on prophylactic and therapeutic immunization against hepatitis B and C viruses.

    Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine·2002
    Same author

    Human milk contains detectable levels of immunoreactive leptin.

    Advances in experimental medicine and biology·2002

    Area of Science:

    • Biochemistry
    • Virology
    • Immunology

    Background:

    • Hepatitis B virus (HBV) infection is a major global health concern.
    • Hepatitis B surface antigen (HBsAg) is a key component of the HBV vaccine and a diagnostic marker.
    • Efficient purification of HBsAg is crucial for vaccine development and serological testing.

    Purpose of the Study:

    • To develop a simple and effective purification protocol for Hepatitis B surface antigen (HBsAg) from human plasma.
    • To assess the purification efficiency using standard biochemical techniques.

    Main Methods:

    • Purification of HBsAg from plasma using a two-step process.
    • Step 1: Polyethylene glycol (PEG) precipitation for initial concentration and purification.
    • Step 2: Batchwise adsorption to hydroxylapatite followed by elution with a specific sodium phosphate buffer.

    Main Results:

    • The initial polyethylene glycol precipitation step yielded a 24-fold purification of HBsAg.
    • An additional 10-fold purification was achieved using hydroxylapatite adsorption and elution.
    • The combined two-step method offers a significantly simplified and effective approach to HBsAg purification.

    Conclusions:

    • A straightforward and efficient two-step purification method for HBsAg from plasma has been established.
    • This protocol enhances HBsAg purity, facilitating its use in vaccine production and diagnostic assays.
    • The method's simplicity and effectiveness make it suitable for broader application in hepatitis B research.

    Related Experiment Videos