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[Differences in permeability for FITC-dextrane in cornea neovascularisation (author's transl)]
Summary
FITC dextran fractions were used to investigate new blood vessels in rat corneas after cauterization. Smaller fractions like FD 3 showed diffusion, while larger ones (FD 40, FD 70, FD 150) had limited or no diffusion in neovascularized areas.
Area of Science:
- Ophthalmology
- Vascular Biology
- Biomedical Imaging
Background:
- Corneal neovascularization is a significant pathological process.
- Understanding vessel permeability is crucial for developing targeted therapies.
- Fluorescein angiography is a standard technique for visualizing corneal vessels.
Purpose of the Study:
- To evaluate the utility of different sizes of FITC dextran fractions for assessing corneal neovascularization.
- To determine the diffusion characteristics of various molecular weights of FITC dextran in newly formed corneal vessels.
Main Methods:
- Corneal cauterization was performed on rats using NaOH.
- Angiographic investigation of neovascularized corneas was conducted 3 weeks post-injury.
- Intravenous administration of different FITC dextran fractions (FD 3, FD 40, FD 70, FD 150) was used.
- FITC dextran diffusion patterns were analyzed angiographically.
Main Results:
- FD 3 demonstrated an angiographic pattern similar to fluorescein sodium, indicating good vascular visualization.
- Minimal diffusion of FD 40 was observed, primarily in the distal terminal loops of neovessels.
- FD 70 and FD 150 showed no significant diffusion within the neovascularized area, suggesting limited permeability.
Conclusions:
- FITC dextran fractions of varying molecular weights can differentiate permeability in corneal neovessels.
- Smaller FITC dextran molecules are more suitable for visualizing the entire neovascular network.
- Larger FITC dextran molecules may be useful for assessing the integrity of the blood-retinal barrier in corneal neovascularization.