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Basic fibroblast growth factor (FGF-2) in mouse tooth morphogenesis

L G Russo¹, P Maharajan, V Maharajan

¹Department of Structure, Function and Biotechnology, Faculty of Veterinary Medicine, University of Naples Federico II, Italy.

Growth Factors (Chur, Switzerland)

|March 20, 1998

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Basic Fibroblast Growth Factor (FGF-2) is crucial for tooth development in mice. FGF-2 expression is highest during odontoblast differentiation and dentin matrix formation, indicating its role in cell differentiation during odontogenesis.

Area of Science:

Developmental Biology
Cell Biology
Biochemistry

Background:

Odontogenesis, the process of tooth formation, involves complex molecular signaling.
Basic Fibroblast Growth Factor (FGF-2) is a key signaling molecule implicated in various developmental processes.

Purpose of the Study:

To investigate the spatio-temporal expression pattern of FGF-2 during mouse odontogenesis.
To elucidate the specific role of FGF-2 in tooth development and cell differentiation.

Main Methods:

Immunohistology was employed on parasagittal sections of mouse embryo heads (13-18 days gestation).
A polyclonal anti-FGF-2 antibody, Streptavidin-Biotin complex-HRP system, and AEC staining were used to detect FGF-2.
Various stages of tooth development, from dental lamina to bell stage, were analyzed.

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Main Results:

FGF-2 expression was absent at the dental lamina stage, minimal at the bud stage, and increased at the cap and bell stages.
Intense staining was observed in stellate reticulum cells, odontoblasts, and the dentin matrix during the bell stage.
Epithelial cells showed faint staining, while enamel matrix was negative for FGF-2.

Conclusions:

FGF-2 plays a significant role in the differentiation of tooth-forming cells, particularly odontoblasts.
FGF-2 is involved in dentin matrix deposition, highlighting its importance in mammalian odontogenesis.
The spatio-temporal distribution of FGF-2 suggests a role in cell differentiation rather than proliferation during tooth development.