Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ontogeny of apoptosis during lung development

M J Kresch1, C Christian, F Wu

  • 1Department of Pediatrics, University of Connecticut School of Medicine, Farmington 06030-2948, USA.

Pediatric Research
|March 20, 1998
PubMed
Summary

Apoptosis, programmed cell death, occurs throughout rat lung development. Its incidence dramatically increases around birth before declining, indicating a crucial role in lung maturation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The effect of an intervention to promote isoniazid preventive therapy on leadership and management abilities.

Public health action·2024
Same author

Association of pectoralis muscle area on computed tomography with airflow limitation severity and respiratory outcomes in COPD: A population-based prospective cohort study.

Pulmonology·2023
Same author

["Leverage pry-off method" for effective prevention of thermal injury during microwave ablation of benign thyroid nodules].

Nan fang yi ke da xue xue bao = Journal of Southern Medical University·2023
Same author

[Epidemiological investigation on the local epidemic situation in Zhengzhou High-Tech Zone caused by SARS-CoV-2 Delta variant].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2023
Same author

[Treatment of trastuzumab-resistant HER2-positive metastatic breast cancer: a case report].

Zhonghua zhong liu za zhi [Chinese journal of oncology]·2022
Same author

[Research progress of biomarkers in steroid-resistant nephrotic syndrome in children].

Zhonghua er ke za zhi = Chinese journal of pediatrics·2022

Area of Science:

  • Developmental Biology
  • Cell Biology
  • Pulmonology

Background:

  • Apoptosis is vital in embryogenesis but its role in lung development remains unclear.
  • Understanding apoptosis ontogeny is crucial for comprehending lung development.

Purpose of the Study:

  • To investigate the occurrence and developmental timeline of apoptosis during rat lung development.
  • To characterize the spatial, temporal, and cell-specific patterns of apoptosis in the developing lung.

Main Methods:

  • Studied apoptosis in vivo in rat lungs from fetal to postnatal stages (14-18 days gestation, birth, and days 1-10 postnatal).
  • Assessed apoptosis using electron microscopy and terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay.
  • Validated findings using fetal rat lung explants cultured in vitro.

Main Results:

  • Apoptosis observed in mesenchymal cells in early fetal lung (14-16 weeks gestation).
  • Both mesenchymal and epithelial apoptosis detected in late fetal lung (18 weeks gestation).
  • A significant 14-fold increase in apoptosis occurred at birth and the first postnatal day (9-12% of cells), followed by a rapid decrease to fetal levels by postnatal day 2-10.

Conclusions:

  • Apoptosis is a spatially, temporally, and cell-specific process during lung development.
  • A marked surge in apoptotic cell numbers occurs immediately after birth, highlighting its critical role in postnatal lung remodeling.

Related Experiment Videos