[Spectrophotometric study of the interaction of single-stranded RNA with tilorone]

The spectrophotometric study of complex formation between phage MS2 single-stranded RNA and tilorone has been carried out. It was found that with components concentration forming "strong linkage" the absorption spectra of RNA-tilorone complex in UV-region experience hyperchromic and bathochromic shifts; besides, a new absorption maximum appears at 280 nm. The changes of spectral properties have been also detected in the long-waved region of the complex spectra. The conclusion has been made that in the course of single-stranded RNA with tilorone interactions a complex formation takes place due to interacalation between spontaneously appeared two-stranded regions in the structure of a single-stranded RNA and tilorone; which component stabilizes these structures and assures there by the previously described interferonogenic activity of a single-stranded RNA--tilorone complexes.