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Related Experiment Videos

Circulating CD34-expressing cells: German Proficiency Testing Survey

S Serke1, D Huhn

  • 1Department of Internal Medicine, Virchow-Klinikum, Humboldt-University, Berlin, Germany.

Journal of Hematotherapy
|March 21, 1998
PubMed
Summary
This summary is machine-generated.

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Accurate CD34+ cell counts are vital for stem cell transplantation. Proficiency testing using engineered samples demonstrates reliable external quality assessment for CD34-expressing cell enumeration.

Area of Science:

  • Hematology
  • Immunology
  • Clinical Diagnostics

Background:

  • Accurate enumeration of CD34-expressing cells is crucial for hematopoietic stem cell transplantation.
  • CD34+ cell quantification is a validated diagnostic parameter in clinical practice.

Purpose of the Study:

  • To assess the reliability of external quality assessment (EQA) surveys for CD34+ cell enumeration.
  • To evaluate the utility of engineered samples for proficiency testing in flow cytometry.

Main Methods:

  • A proficiency testing survey was conducted in Germany involving 58 participants.
  • Commercially available, stabilized myeloblastic leukemia cells (KG1a) were spiked into normal blood samples at defined concentrations.
  • Report forms were analyzed from 44 participants who returned completed data.

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Main Results:

  • Mean CD34+ cell percentages were 0.49% (predicted 0.5%) and 0.29% (predicted 0.25%).
  • Coefficients of variation were 57% for sample A and 83% for sample B.
  • Engineered samples showed acceptable performance for EQA.

Conclusions:

  • Engineered samples using normal blood and stabilized KG1a cells are suitable for EQA of CD34+ cell enumeration.
  • These materials provide a reliable basis for external quality assessment surveys in flow cytometry.
  • Standardized EQA is essential for ensuring accurate CD34+ cell counts in transplantation settings.