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Related Experiment Videos

Temperature dependence of outer hair cell nonlinear capacitance

J Santos-Sacchi1, G Huang

  • 1Section of Otolaryngology, Yale University School of Medicine, New Haven, CT 06510, USA. joseph.santos-sacchi@yale.edu

Hearing Research
|March 21, 1998
PubMed
Summary

Temperature affects outer hair cell (OHC) voltage-dependent functions, shifting peak capacitance negatively with cooling. This suggests OHCs

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Area of Science:

  • Auditory Neuroscience
  • Cellular Physiology
  • Biophysics

Background:

  • Outer hair cells (OHCs) are crucial for hearing, exhibiting motility essential for cochlear amplification.
  • Temperature is known to influence auditory system function, but its direct impact on OHC electrophysiology is not fully elucidated.

Purpose of the Study:

  • To investigate the temperature dependence of motility-related gating currents and capacitance in OHCs.
  • To elucidate the biophysical mechanisms by which temperature influences OHC function and, consequently, cochlear physiology.

Main Methods:

  • Whole-cell voltage clamp technique was employed to measure OHC gating current and capacitance.
  • Experiments were conducted across a range of temperatures to assess temperature-dependent shifts.

Main Results:

  • Temperature changes induced significant voltage-axis shifts in OHC voltage-dependent functions.
  • A decrease in temperature resulted in a negative shift of Vpkcm (voltage at peak capacitance) by 19.2 mV per 10°C.
  • Gating current kinetics exhibited mild temperature dependence, with a Q10 value of approximately 1.5.

Conclusions:

  • Temperature significantly modulates OHC voltage-dependent gating and capacitance, impacting their electromechanical properties.
  • Alterations in lateral membrane viscoelastic properties are proposed as the mechanism for temperature effects on OHCs.
  • These findings provide a cellular basis for understanding temperature-dependent effects on in vivo cochlear physiology and hearing.

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