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PCR methods for rapid identification and characterization of Actinobacillus seminis strains
S Appuhamy1, J G Coote, J C Low
1Division of Infection and Immunity, University of Glasgow, Scotland, United Kingdom.
Abstract:
Twenty-four isolates of Actinobacillus seminis were typed by PCR ribotyping, repetitive extragenic palindromic element (REP)-based PCR, and enterobacterial repetitive intergenic consensus (ERIC)-based PCR. Five types were distinguished by REP-PCR, and nine types were distinguished by ERIC-PCR. PCR ribotyping produced the simplest pattern and could be useful for identification of A. seminis and for its differentiation from related species. REP- and ERIC-PCR could be used for strain differentiation in epidemiological studies of A. seminis.
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