Ultraweak chemiluminescence arising for glutaraldehyde-induced cross-linking reactions of biomolecules

Chemiluminescence arising from cross-linking reactions of glutaraldehyde was examined. Our findings indicate that: (i) new reactions not yet described in the literature may be responsible for a part of the observed emissions; and (ii) this chemiluminescence may offer a new way of optimizing procedures of fixing biological materials prepared for microscopic examinations.