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A murine TSPY

T Vogel1, H Boettger-Tong, I Nanda

  • 1Institute of Human Genetics, Medizinische Hochschule Hannover, Germany.

Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology
|March 24, 1998
PubMed
Summary
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The mouse Y-chromosome TSPY gene, homologous to human and bovine sequences, appears non-functional and single-copy. TSPY likely lost function during rodent evolution, unlike in primates and artiodactyls.

Area of Science:

  • Genetics
  • Evolutionary Biology
  • Mammalian Reproduction

Background:

  • The TSPY gene, found on the Y chromosome, is known in humans and bovines.
  • TSPY exhibits variations in copy number and functionality across different mammalian lineages.

Purpose of the Study:

  • To investigate the presence, structure, and functionality of the TSPY gene in Mus musculus (mice).
  • To compare murine TSPY with its homologs in other species to understand its evolutionary trajectory.

Main Methods:

  • Isolation of TSPY homologous sequences from mouse testicular cDNA.
  • Polymerase chain reaction (PCR) amplification of the TSPY gene from mouse genomic DNA.
  • Sequence comparison of murine, human, and bovine TSPY exons.

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Main Results:

  • A nearly full-length TSPY gene was amplified from mouse genomic DNA, but it appears non-functional.
  • Unlike primates and artiodactyls, murine Tspy is present as a single copy.
  • Murine Tspy is located on the Yp chromosome, in the same syntenic group as human TSPY.
  • Sequence analysis suggests TSPY became non-functional during rodent evolution.

Conclusions:

  • The mouse TSPY gene is a non-functional, single-copy gene located on the Yp chromosome.
  • The loss of TSPY functionality in rodents represents a divergence from primate and artiodactyl TSPY evolution.
  • Rodent TSPY evolution is characterized by gene inactivation, contrasting with its moderate repetitiveness in other lineages.