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Updated: Aug 10, 2026

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An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium
Published on: July 22, 2011
PCR single strand conformational polymorphism can be used to detect the gene encoding SHV-7 extended-spectrum
F H M'Zali1, J Heritage, D M Gascoyne-Binzi
1Department of Microbiology, University of Leeds, UK.
The Journal of Antimicrobial Chemotherapy
|March 25, 1998
Abstract:
The PCR single strand conformational polymorphism (PCR-SSCP) technique described to identify mutants of the SHV beta-lactamases was extended to identify an SHV-7 type beta-lactamase. This was found in a strain of Klebsiella pneumoniae, the first recorded isolate in the UK to produce this type of enzyme. We also demonstrate that PCR-SSCP can be used to identify more than one SHV beta-lactamase gene in a single strain.

