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[A monoclonal antibody panel for the intratypic differentiation of type-2 polioviruses]
V P Shyrobokov1, I V Nikolaienko, L V Kopanytsia
1Bogomoletz National Medical University, Kyiv, Ukraine.
A panel of murine hybridomas producing monoclonal antibodies is obtained by means of hybridization of splenocytes with Sp-2/0 myelomas. They are able to differentiate between vaccine and virulent poliovirus type II strains in the reaction of neutralization. The ability of monoclonal antibodies to differentiate polioviruses of different origins was investigated on reference strains as well as on the field poliovirus isolates compared to those obtained using the monoclonal antibodies from Pasteur Institute, polymerase chain reaction (PCR) test and ELISA tests with cross-adsorbent sera as recommended by World Health Organization. It is demonstrated that 4 of 6 obtained monoclonal antibodies allow precise differentiation of the field poliovirus isolates with the reliability comparable to PCR or ELISA test with cross-adsorbent sera. We also show that the obtained panel of monoclonal antibodies can be used for studying antigenic similarity in poliovirus vaccine type II strains.
A panel of murine hybridomas producing monoclonal antibodies is obtained by means of hybridization of splenocytes with Sp-2/0 myelomas. They are able to differentiate between vaccine and virulent poliovirus type II strains in the reaction of neutralization. The ability of monoclonal antibodies to differentiate polioviruses of different origins was investigated on reference strains as well as on the field poliovirus isolates compared to those obtained using the monoclonal antibodies from Pasteur Institute, polymerase chain reaction (PCR) test and ELISA tests with cross-adsorbent sera as recommended by World Health Organization. It is demonstrated that 4 of 6 obtained monoclonal antibodies allow precise differentiation of the field poliovirus isolates with the reliability comparable to PCR or ELISA test with cross-adsorbent sera. We also show that the obtained panel of monoclonal antibodies can be used for studying antigenic similarity in poliovirus vaccine type II strains.