Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Frequency shift analysis of tetracycline

S A Nabi1, E S Abu-Nameh, M I Helaleh

  • 1Department of Chemistry, Aligarh Muslim University, India.

Acta Poloniae Pharmaceutica
|May 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Assessment of proline function in higher plants under extreme temperatures.

Plant biology (Stuttgart, Germany)·2023
Same author

Design of a compact and broadband terahertz polarization splitter based on gradient dual-core photonic crystal fiber.

Applied optics·2020
Same author

Development of AhMITE1 markers through genome-wide analysis in peanut (Arachis hypogaea L.).

BMC research notes·2018
Same author

Exploring plant growth-promotion actinomycetes from vermicompost and rhizosphere soil for yield enhancement in chickpea.

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]·2016
Same author

Marker-trait association study for protein content in chickpea (Cicer arietinum L.).

Journal of genetics·2015
Same author

Plastic fiber design for THz generation through wavelength translation.

Optics letters·2015

This study introduces a new spectrophotometric method for quantifying tetracycline using DMSO-induced wavelength shifts. The method accurately measures tetracycline in water and spiked urine samples at low concentrations.

Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Pharmaceutical Analysis

Background:

  • Tetracycline is a widely used antibiotic.
  • Accurate quantification of tetracycline is crucial for therapeutic drug monitoring and quality control.
  • Existing analytical methods may require complex procedures or expensive equipment.

Purpose of the Study:

  • To develop a simple and sensitive spectrophotometric method for tetracycline determination.
  • To investigate the interaction between tetracycline and DMSO for analytical purposes.
  • To validate the method for real-world sample matrices like urine.

Main Methods:

  • Spectrophotometric analysis of tetracycline in aqueous solutions.
  • Utilizing the wavelength shift of absorption maxima (λmax 360 nm) caused by hydrogen bonding with DMSO.

Related Experiment Videos

  • Quantification within the concentration range of 0.04 to 0.18 ppm.

    • Main Results:

    • A distinct wavelength shift in tetracycline's absorption maximum was observed due to DMSO.
    • The developed spectrophotometric method demonstrated good sensitivity for tetracycline detection.
    • The method was successfully applied to determine tetracycline in spiked human urine samples.

    Conclusions:

    • The described spectrophotometric method offers a reliable approach for tetracycline quantification.
    • The interaction with DMSO provides a basis for a straightforward analytical technique.
    • The method's applicability to urine samples highlights its potential for clinical and forensic analysis.