Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Laser-based spectroscopic methods in tissue characterization

K Svanberg1, C af Klinteberg, A Nilsson

  • 1Department of Oncology, Lund University Hospital, Sweden. katarina.svanberg@fysik.lth.se

Annals of the New York Academy of Sciences
|March 25, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

How carbon monoxide adsorbs in different sites.

Physical review letters·2000
Same author

Alkaline sphingomyelinases and ceramidases of the gastrointestinal tract.

Chemistry and physics of lipids·2000
Same author

Wheezing following lower respiratory tract infections with respiratory syncytial virus and influenza A in infancy.

Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology·2000
Same author

Allopurinol in addition to 5-aminosalicylic acid based drugs for the maintenance treatment of ulcerative colitis.

Alimentary pharmacology & therapeutics·2000
Same author

Partitioning of peptides and recombinant protein-peptide fusions in thermoseparating aqueous two-phase systems: effect of peptide primary structure.

Journal of chromatography. B, Biomedical sciences and applications·2000
Same author

Recombining germline-derived CDR sequences for creating diverse single-framework antibody libraries.

Nature biotechnology·2000
Same journal

Multiomics Profiling During Autoimmune Demyelination Highlights a Complex Regulatory Role for Ataxin-1 in B Cells.

Annals of the New York Academy of Sciences·2026
Same journal

Global Trends in Light Pollution and Their Relationship With Socioeconomic Factors.

Annals of the New York Academy of Sciences·2026
Same journal

Wired for Corruption: Inter-Brain Synchrony Encodes Bribery-Related Value Information and Predicts Bribery Agreement.

Annals of the New York Academy of Sciences·2026
Same journal

LM-YOLO: A Lightweight Multi-Scale Enhanced Model for Forest Smoke Detection Using Unmanned Aerial Vehicles.

Annals of the New York Academy of Sciences·2026
Same journal

Polyrhythm Perception and Production: A Scoping Review.

Annals of the New York Academy of Sciences·2026
Same journal

DARTS-CNN-BiLSTM: Intelligent Fault Diagnosis for Computer Numerical Control Machine Tool Feed System.

Annals of the New York Academy of Sciences·2026
See all related articles

Laser-induced fluorescence spectroscopy effectively characterizes tumors using delta-amino levulinic acid (ALA) to induce protoporphyrin IX (PpIX). This technique aids in precise tumor demarcation across various human cancers.

Area of Science:

  • Medical Physics
  • Oncology
  • Biophotonics

Background:

  • Accurate tumor characterization is crucial for effective cancer treatment.
  • Laser-induced fluorescence (LIF) offers a promising non-invasive diagnostic tool.
  • Tumor-localizing substances can enhance fluorescence signals for better detection.

Purpose of the Study:

  • To develop and evaluate laser-based spectroscopic techniques for tumor tissue characterization.
  • To investigate the utility of delta-amino levulinic acid (ALA) for protoporphyrin IX (PpIX) induction in tumors.
  • To assess the efficacy of LIF for tumor demarcation in various human malignancies.

Main Methods:

  • Sensitization of tumor tissue using delta-amino levulinic acid (ALA) administered topically, orally, or intravenously.

Related Experiment Videos

  • Monitoring of autofluorescence and protoporphyrin IX (PpIX)-related fluorescence signals.
  • In vivo time-integrated laser-induced fluorescence measurements using a point fluorosensor and multicolor fluorescence imaging system.

    • Main Results:

    • Calculated tumor demarcation functions for various human malignant tumors, including bladder, prostate, head and neck, breast, and gastrointestinal cancers.
    • Examined colon tumors and esophageal tumors/dysplastic lesions, including in patients with Barrett's esophagus.
    • Demonstrated the feasibility of LIF for in vivo tumor detection and characterization across different clinical specialties.

    Conclusions:

    • Laser-based spectroscopic techniques utilizing ALA-induced PpIX fluorescence are effective for tumor tissue characterization.
    • The developed methods show potential for precise tumor demarcation in a wide range of human cancers.
    • LIF spectroscopy represents a valuable tool for improving cancer diagnosis and surgical guidance.