The effects of MK-801 on spatial working memory and within-session spatial learning

The present study investigated the effects of the NMDA channel blocker MK-801 (0.05, 0.10, and 0.15 mg/kg) on a task that allows for the assessment of both spatial working memory and within-session spatial learning. During the first trial of each day, subjects were shown the spatial location of a food reward on a six-arm radial-arm maze. During nine subsequent free-choice trials, subjects were reinforced for returning to that same spatial location. The location of the food reward varied across days. Thus, choosing correctly on any given trial required subjects to remember where food had been received during the previous trials of that day. The effects of MK-801 on working memory were assessed by analyzing the overall number of errors committed during the nine free-choice trials of each day. The effects of MK-801 on within-session learning were assessed by comparing the number of errors committed during the first three trials of each day to the number of errors committed during the last three trials of each day. Only the highest dose of MK-801 tested (0.15 mg/kg) impaired spatial working memory. No dose of MK-801 impaired the ability of subjects to acquire spatial information within a given session. The failure of MK-801 to impair within-session spatial learning stands in contrast to the well-known effects of MK-801 on spatial learning measured across days. Thus, when coupled with previous research, the findings of the present study further suggest that the NMDA receptor plays a role in the long-term, but not short-term, storage of spatial information.