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Site-directed mutagenesis of the Proteus mirabilis glutathione transferase B1-1 G-site
E Casalone1, N Allocati, I Ceccarelli
1Dipartimento di Scienze Biomediche, Università G. D'Annunzio, Chieti, Italy.
FEBS Letters
|March 25, 1998
Abstract:
In order to investigate the roles of near N-terminus Tyr, Cys, and Ser residues in the activity of bacterial glutathione transferase (GSTB1-1) site-directed mutagenesis was used to replace the following residues: Tyr-4, Tyr-5, Ser-9, Cys-10, Ser-11, and Ser-13. The results presented here show that, unlike all other alpha, mu, pi, theta and sigma classes of glutathione transferases so far investigated, GSTB1-1 does not utilise any Tyr, Ser or Cys residue to activate glutathione. These results also suggest that the bacterial glutathione transferases may require classification into their own class.