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Steroid formation in osteoblast-like cells

H Saito1, T Yanaihara

  • 1Department of Obstetrics and Gynaecology, Showa University School of Medicine, Tokyo, Japan.

The Journal of International Medical Research
|March 26, 1998
PubMed
Summary

Postmenopausal bone loss can be prevented by estrogen replacement. Osteoblast-like cells can produce potent estrogens and androgens from circulating steroids, suggesting bone

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Area of Science:

  • Endocrinology
  • Bone Biology
  • Cellular Metabolism

Background:

  • Estrogen replacement therapy is vital for preventing bone mass reduction in postmenopausal women.
  • Sex steroids play a critical role in bone metabolism for both sexes.
  • Steroid-converting enzyme activities have been observed in osteoblast and osteoblast-like cells in vitro.

Purpose of the Study:

  • To investigate the expression of key steroid-converting enzymes and hormone receptors in human osteoblast-like cell lines.
  • To determine if osteoblast-like cells can synthesize biologically active sex steroids.
  • To elucidate the role of bone in facilitating hormonal action.

Main Methods:

  • Utilized reverse transcription-polymerase chain reaction (RT-PCR) to analyze messenger ribonucleic acid (mRNA) expression.
  • Assessed mRNA levels for aromatase, steroid sulfatase, 5 alpha-reductase, 17 beta-hydroxysteroid dehydrogenase (17 beta-HSD), and 3 beta-HSD.
  • Quantified mRNA expression for estrogen, androgen, and progesterone receptors in MG 63 and HOS cell lines.

Main Results:

  • Both MG 63 and HOS human osteoblast-like cell lines expressed mRNA for all investigated steroid-converting enzymes without induction.
  • Estrogen, androgen, and progesterone receptor mRNAs were also detected in both cell lines.
  • These findings indicate intrinsic steroidogenic capabilities within these bone cells.

Conclusions:

  • Osteoblast-like cells possess the intrinsic capacity to synthesize potent estrogens and androgens from circulating steroid precursors.
  • Bone tissue may play a significant role in local hormone production and action.
  • This cellular steroidogenesis in bone could have implications for bone health and hormonal therapies.

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