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Updated: Aug 16, 2026

An Improved Method for the Preparation of Type I Collagen From Skin
Published on: January 21, 2014
Age-related changes in type-I collagen synthesis in human eyelid skin
C M DeBacker1, A M Putterman, L Zhou
1Duke University Eye Center, Durham, North Carolina 27710, USA.
Purpose:
This study was to determine whether age-related decrements in type I collagen synthesis occur in human eyelid skin.
Methods:
Using an antibody to procollagen I, we investigated collagen synthetic activity in skin removed for cosmetic purposes from 10 white patients between the ages of 4 and 77 years. Eleven masked referees graded the immunostaining on a scale of 1 (most intense) to 10 (least intense).
Results:
The multiple range test for rank by group demonstrated more intense staining in younger patients compared with older patients. An average correlation coefficient of 0.8432 (p < 0.05) existed between each of the referee's rankings.
Conclusion:
Type I collagen synthesis diminishes with age in eyelid skin.
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