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Development of a polymerase chain reaction assay for Mycoplasma salivarium by using the nucleotide sequence within
T Yoshikawa1, K Shibata, M Kaga
1Department of Oral Bacteriology, Hokkaido University School of Dentistry, Sapporo, Japan.
FEMS Microbiology Letters
|March 26, 1998
Abstract:
A polymerase chain reaction assay for a 278-nucleotide DNA fragment within aminopeptidase My gene of Mycoplasma salivarium was developed. The assay amplified M. salivarium DNA, but did not amplify DNAs of other mollicutes, bacteria and mammalian cells. The detection limit of the assay was 10 fg of DNA, approximately equivalent to 10 organisms.