Microbial contamination of three-way taps on arterial lines
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Updated: Oct 30, 2026

Antimicrobial Characterization of Advanced Materials for Bioengineering Applications
Published on: August 4, 2018
W M Widdowson1, L Walker, J H Havill
1Intensive Care Unit, Waikato Hospital, Hamilton, New Zealand.
Abstract:
Arterial lines with three-way taps are used to measure blood pressure and aspirate blood, and are a potential source of catheter-related sepsis. Swabs were taken daily from 118 three-way taps on 98 arterial lines in a general intensive care unit. Infusion lines were changed weekly but arterial cannulae were not changed routinely. An overall contamination rate of 24.6% was found with the predominant organism being coagulase negative staphylococcus. The three-way taps became increasingly contaminated with time but this was shown to be unrelated to the manipulation rates. Blood culture organisms in those showing contamination of the three-way taps showed no relationship to the bacteria causing the contamination.
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