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Diurnal rhythm of hypophyseal cells in mich. II. Pars intermedia
Abstract:
By the use of Mac Conaill's lead hematoxylin, periodic acid and Schiff's reagent (PAS, PbH-positive and PAS-positive cells were distinguished in the pars intermedia of the hypophysis in mice. Nuclear volume in PbH-positive and PAS-positive cells in the pars intermedia of the hypophysis in male and female mice under conditions of 12 h light and 12 h darkness shows distinct diurnal rhythmicity. Maximum nuclear volume in PbH-positive cells of the pars intermedia in both sexes was observed at 1800 h, and minimum at 2400 h. In the Pas-positive cells in females maximum nuclear volume was observed at 600 h, and minimum at 2400 h. In males maximal nuclear volume in these cells appears at 2400 h, and minimum at 1800 h. Maximum number of vacuoles in the nuclei of PbH-positive cells in the pars intermedia in both sexes appeared at 1800 h, and minimum at 2400 h. Maximum numbers of vacuoles in the nuclei of PAS-positive cells in females was noted at 1200 h, and minimum at 2400 h. In males the maximum number of vacuoles appeared at 600 h, and minimum at 1200 h. Differences in the number of vacuoles in the nuclei of PAS-positive cells between males and females were also noted.
Insights
The pars intermedia of the mouse hypophysis exhibits diurnal rhythmicity in nuclear volume and vacuole formation within lead hematoxylin (PbH)-positive and periodic acid-Schiff (PAS)-positive cells. These cellular changes vary between sexes and throughout the light-dark cycle.
Area of Science:
- Endocrinology
- Cell Biology
- Chronobiology
Background:
- The pars intermedia of the hypophysis plays a role in hormone secretion.
- Cellular characteristics within the pars intermedia may exhibit temporal variations.
- Understanding diurnal rhythms is crucial for interpreting physiological processes.
Purpose of the Study:
- To investigate diurnal rhythmicity in cellular morphology of the mouse hypophysis pars intermedia.
- To differentiate and analyze PbH-positive and PAS-positive cells.
- To compare nuclear volume and vacuole formation between sexes.
Main Methods:
- Utilized Mac Conaill's lead hematoxylin and periodic acid-Schiff (PAS) staining techniques.
- Distinguished PbH-positive and PAS-positive cells in the pars intermedia.
- Quantified nuclear volume and counted nuclear vacuoles in male and female mice under a 12h light/12h dark cycle.
Main Results:
- Both PbH-positive and PAS-positive cells showed distinct diurnal rhythms in nuclear volume and vacuole number.
- Maximum nuclear volume in PbH-positive cells occurred at 1800h, minimum at 2400h.
- PAS-positive cells displayed sex-specific rhythms in nuclear volume and vacuole counts, with notable differences between males and females.
Conclusions:
- The pars intermedia of the mouse hypophysis exhibits significant diurnal rhythmicity in cellular characteristics.
- Nuclear volume and vacuole formation are influenced by the time of day and sex.
- These findings highlight the dynamic nature of the hypophysis and its response to circadian cycles.