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Varying effects of ethanol on transfected cell lines
1Ernest Gallo Clinic and Research Center, University of California, San Francisco 94110, USA.
Alcoholism, Clinical and Experimental Research
|March 26, 1998
Abstract:
The use of transfected cell lines has provided a powerful approach to study the functions of transfected proteins. This approach has also proven useful to determine the effects of acute and chronic ethanol exposure on particular proteins. We show here, however, that the effects of ethanol on transfected proteins vary between transiently transfected cells and stably transfected cell lines. Moreover, we found that the effect of ethanol on a particular protein depends on the plasmid vectors used for the transfection.