Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Androgens alter B cell development in normal male mice

S M Viselli1, K R Reese, J Fan

  • 1Division of Rheumatology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA.

Cellular Immunology
|March 26, 1998
PubMed
Summary

Androgens directly influence B cell numbers in bone marrow. Castration increases B cells, while hormone replacement reverses this effect, indicating a direct role for androgens in B cell regulation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

miR-216a rescues dexamethasone suppression of osteogenesis, promotes osteoblast differentiation and enhances bone formation, by regulating c-Cbl-mediated PI3K/AKT pathway.

Cell death and differentiation·2015
Same author

Distributions of topological observables in inclusive three- and four-jet events in pp collisions at [Formula: see text][Formula: see text].

The European physical journal. C, Particles and fields·2015
Same author

Evidence for Collective Multiparticle Correlations in p-Pb Collisions.

Physical review letters·2015
Same author

Constraints on parton distribution functions and extraction of the strong coupling constant from the inclusive jet cross section in pp collisions at [Formula: see text][Formula: see text].

The European physical journal. C, Particles and fields·2015
Same author

Search for a standard model Higgs boson produced in association with a top-quark pair and decaying to bottom quarks using a matrix element method.

The European physical journal. C, Particles and fields·2015
Same author

Search for dark matter, extra dimensions, and unparticles in monojet events in proton-proton collisions at [Formula: see text][Formula: see text].

The European physical journal. C, Particles and fields·2015

Area of Science:

  • Immunology
  • Endocrinology
  • Cell Biology

Background:

  • Castration in male mice causes spleen enlargement and increased B cell populations.
  • Androgens may influence B cells indirectly through other organs, as spleens lack androgen receptors.

Purpose of the Study:

  • To investigate the direct role of androgens in modulating B cell populations within the bone marrow.
  • To determine if the thymus is involved in androgen-mediated B cell expansion.

Main Methods:

  • Confirmed B cell expansion in bone marrow after castration and demonstrated reversibility with androgen replacement.
  • Assessed the impact of thymectomy on B cell expansion in castrated mice.
  • Utilized immunoblotting and ligand binding assays to detect androgen receptors in B cells and bone marrow stromal cells.

Related Experiment Videos

Main Results:

  • Castration led to an expansion of bone marrow B cells, which was reversible with androgen therapy.
  • Thymectomy did not alter B cell expansion in castrated mice, suggesting the thymus is not a key mediator.
  • Androgen receptors were detected in immature B cells and bone marrow stromal cells.

Conclusions:

  • Androgens appear to directly modulate B cell populations within the bone marrow.
  • The thymus is unlikely to be the primary site for androgen-mediated effects on B cell expansion.