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Development of a scintillation proximity assay for peroxisome proliferator-activated receptor gamma ligand binding

J S Nichols1, D J Parks, T G Consler

  • 1Department of Molecular Biochemistry, Glaxo Wellcome, Inc., Research Triangle Park, North Carolina 27709-3398, USA.

Analytical Biochemistry
|March 26, 1998
PubMed

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...