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Sendai viruses with altered P, V, and W protein expression
C Delenda1, G Taylor, S Hausmann
1Department of Genetics and Microbiology, University of Geneva School of Medicine, CMU, Geneva, Switzerland.
Virology
|March 26, 1998
Summary
Sendai virus P gene editing creates P, V, and W proteins. Altering this editing affects viral virulence in mice, with V and W proteins being crucial for pathogenesis in C57BI/6 mice.
Area of Science:
- Virology
- Molecular Biology
- Immunology
Background:
- Sendai virus P gene mRNA editing produces P, V, and W proteins.
- The relative roles of these proteins in viral pathogenesis are not fully understood.
Purpose of the Study:
- To investigate the impact of altered P gene mRNA editing on Sendai virus replication and virulence.
- To elucidate the specific roles of V and W proteins in viral pathogenesis.
Main Methods:
- Generation of recombinant Sendai viruses with modified P gene mRNA editing.
- In vitro characterization of viral replication and macromolecule accumulation.
- In vivo assessment of viral competitiveness and virulence in mouse models (C57BI/6 and ICR).
Main Results:
- Recombinant viruses with altered editing grew similarly in eggs and cell lines.
- Relative competitive advantage varied, with V-stop and wild-type viruses outperforming others.
- Virulence in mice differed significantly: rSeV-swap/8 was highly virulent, rSeV-delta 6A was avirulent, and rSeV-Vstop showed differential virulence based on mouse strain.
Conclusions:
- Sendai virus P, V, and W protein expression is modulated by mRNA editing.
- Both V and W proteins are sufficient for pathogenesis in C57BI/6 mice.
- W protein partially substitutes for V protein in ICR mouse pathogenesis, indicating strain-specific roles.