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Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
Centrifugation01:05

Centrifugation

Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

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Related Experiment Video

Updated: Jul 15, 2026

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
09:45

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

Continuous dielectrophoretic separation of cell mixtures

H A Pohl, K Kaler

    Cell Biophysics
    |March 1, 1979
    PubMed
    Summary

    Stream-centered dielectrophoresis effectively separated mixtures of algae and yeast cells. This method offers a promising technique for continuous cell separation across various biological applications.

    Area of Science:

    • Biophysics
    • Cell Biology
    • Microfluidics

    Background:

    • Continuous cell separation is crucial for biological research and applications.
    • Dielectrophoresis (DEP) offers a non-invasive method for manipulating and separating cells.
    • Optimizing DEP parameters is essential for achieving efficient separation of diverse cell types.

    Purpose of the Study:

    • To demonstrate the efficacy of stream-centered dielectrophoresis for separating mixtures of microorganisms.
    • To determine optimal frequencies for separating specific algae and yeast cell mixtures.
    • To explore the adaptability of the technique for cells requiring different suspension conditions.

    Main Methods:

    • Utilized stream-centered dielectrophoresis (DEP) for continuous separation.

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    Preparation of Primary Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases by Centrifugal Elutriation

    Published on: November 10, 2017

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    Last Updated: Jul 15, 2026

    Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
    09:45

    Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

    Published on: February 4, 2011

    Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis
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    Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis

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    Preparation of Primary Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases by Centrifugal Elutriation
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    Preparation of Primary Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases by Centrifugal Elutriation

    Published on: November 10, 2017

  • Tested three distinct cell mixtures: Chorella vulgaris/Netrium digitus, Ankistrodesmus falcatus/Staurastrum gracile, and Saccharomyces cerevisiae/Netrium digitus.
  • Operated within a frequency range of 0.01-32 MHz in low-conductivity suspensions.

    • Main Results:

    • Achieved maximal separation for the tested cell mixtures at specific frequencies: 100 kHz, 600 kHz, and 2.0 MHz.
    • Demonstrated successful continuous separation of live algae and yeast cells.
    • Identified the technique's applicability to low-conductivity suspensions.

    Conclusions:

    • Stream-centered dielectrophoresis is a viable method for continuous separation of microorganisms.
    • The technique can be adapted for cells requiring higher osmolarity by using nonionic solutes.
    • This method holds potential for various cell separation applications in biotechnology and research.