Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sustained neurotransmitter release: new molecular clues

L Brodin1, P Löw, H Gad

  • 1Department of Neuroscience, Nobel Institute for Neurophysiology, Karolinska Institutet, Stockholm, Sweden.

The European Journal of Neuroscience
|March 28, 1998
PubMed
Summary

Synaptic vesicle replenishment is crucial for high-frequency nerve impulse transmission. Two key mechanisms, clathrin-mediated endocytosis and reserve pool mobilization, ensure rapid vesicle recycling to prevent synaptic failure.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Perirenal fat surface area as a risk factor for perioperative difficulties and 30-day postoperative complications in elective colon cancer surgery.

Colorectal disease : the official journal of the Association of Coloproctology of Great Britain and Ireland·2018
Same author

High birth weight was associated with increased radial artery intima thickness but not with other investigated cardiovascular risk factors in adulthood.

Acta paediatrica (Oslo, Norway : 1992)·2018
Same author

A comparison of patients' and physicians' assessments of disease activity using the Swedish version of the Systemic Lupus Activity Questionnaire.

Scandinavian journal of rheumatology·2017
Same author

Validation and development of MTH1 inhibitors for treatment of cancer.

Annals of oncology : official journal of the European Society for Medical Oncology·2016
Same author

Overexpression of the scaffold WD40 protein WRAP53β enhances the repair of and cell survival from DNA double-strand breaks.

Cell death & disease·2016
Same author

Erratum to: increased and early lipolysis in children with long-chain 3-hydroxyacyl-CoA dehydrogenase (LCHAD) deficiency during fast.

Journal of inherited metabolic disease·2014

Area of Science:

  • Neuroscience
  • Cell Biology
  • Synaptic Transmission

Background:

  • Chemical synapses transmit nerve impulses via exocytosis of synaptic vesicles.
  • High-frequency transmission necessitates rapid replenishment of these vesicles to avoid synaptic failure.
  • Clathrin-mediated endocytosis is a known pathway for synaptic vesicle recycling.

Purpose of the Study:

  • To investigate the mechanisms underlying rapid synaptic vesicle replenishment.
  • To determine the roles of clathrin-mediated recycling and reserve pool mobilization in synaptic transmission.
  • To understand how different synaptic activity patterns influence vesicle replenishment efficacy.

Main Methods:

  • Molecular perturbation studies were employed to investigate synaptic vesicle dynamics.

Related Experiment Videos

  • Synapsin perturbation was used to assess the impact on vesicle clusters and synaptic transmission.
  • Comparative analysis of synaptic transmission at high and low frequencies was performed.

    • Main Results:

    • Synaptic vesicle recycling involves clathrin-mediated endocytosis.
    • Mobilization of vesicles from clustered pools allows exocytosis rates to exceed recycling rates.
    • Perturbation of synapsins disrupts vesicle clusters and impairs high-frequency transmission.
    • Both recycling and mobilization are vital for vesicle replenishment, with differing efficacy based on synapse activity.

    Conclusions:

    • Synaptic vesicle replenishment relies on both clathrin-mediated endocytosis and reserve pool mobilization.
    • The balance between these mechanisms is critical for maintaining synaptic function under varying activity levels.
    • Understanding these processes is key to comprehending synaptic plasticity and failure.