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t(2;5) positive lymphoma with peripheral blood involvement
M Chhanabhai1, C Britten, R Klasa
1Department of Pathology, British Columbia Cancer Agency and University of British Columbia, Vancouver, Canada.
Leukemia & Lymphoma
|March 28, 1998
Summary
Two cases of CD30-positive lymphomas with t(2;5) translocation were identified, showing peripheral blood involvement. Reverse transcription-polymerase chain reaction (RT-PCR) proved more sensitive than microscopy for detecting disease in bone marrow and peripheral blood.
Area of Science:
- Hematology
- Oncology
- Molecular Biology
Background:
- CD30-positive lymphomas, including anaplastic large cell lymphoma (ALCL), are often associated with the t(2;5) translocation.
- Peripheral blood (PB) and bone marrow (BM) involvement can occur in these lymphomas, impacting staging and prognosis.
Observation:
- Two cases of CD30-positive lymphomas with t(2;5) translocation and PB involvement are presented.
- Case one, a diffuse large cell lymphoma, showed BM and PB disease detected by CD30 staining and RT-PCR for t(2;5).
- Case two, an ALCL, had abnormal circulating cells in PB with the NPM/ALK fusion product detected by RT-PCR.
Findings:
- Not all CD30-positive and t(2;5)-associated lymphomas exhibit anaplastic morphology.
- CD30 staining in BM biopsies is valuable for pathological staging.
- RT-PCR for t(2;5) is a more sensitive method for detecting disease in PB and BM compared to light microscopy.
Implications:
- Routine CD30 staining and EMA in BM biopsies aid pathological staging.
- The precise role of t(2;5) in defining specific lymphoma subtypes requires further investigation.
- The clinical significance of molecular staging using RT-PCR in ALCL patients warrants further evaluation.