Related Experiment Videos
Ex vivo assessment of lymphocyte antioxidant status using the comet assay
1Boyd Orr Research Centre, University of Aberdeen, Bucksburn, Scotland, UK.
Free Radical Research
|March 28, 1998
Abstract:
Lymphocytes were isolated from volunteers before and after receiving a single supplement of vitamin C, vitamin E or beta-carotene. The lymphocytes were treated with H2O2, and DNA strand breaks were measured by single cell gel electrophoresis (the comet assay). Significant protection against oxidative DNA damage was evident 2-4 h after vitamin C intake, and 18-24 h after consumption of the other antioxidants. Lymphocytes from smokers were more sensitive to DNA damage than those from non-smokers, and they showed at least as great a protective effect with antioxidants.