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Updated: Aug 10, 2026

Electrophoretic Separation of Proteins
Published on: June 12, 2008
Separation of paraproteins from human plasma by membrane chromatography
1Institute of Biochemistry, University of Leipzig, Germany.
Abstract:
Membrane chromatography using a commercially available blotting membrane was performed in a dead-end filtration mode to separate paraproteins from plasma of patients suffering from paraproteinemia. The affinity membrane was found to display distinct specificity to monoclonal IgG1. A dissociation constant (Kd) of 3.2 microM and a maximum binding capacity of 1.43 mg/cm2 IgG1 paraprotein were obtained from the adsorption isotherm of the affinity membrane. The membrane was found to absorb immunoglobulins species-dependently because no binding of immunoglobulins from mouse, rat and rabbit could be observed.
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