The retinal ganglion cells that drive the pupilloconstrictor response in rats

It is well established that the pupillary light reflex (PLR) in rats is mediated by a direct retinal projection to the olivary pretectal nucleus (OPN). Although several authors have commented on the specific subpopulation of retinal ganglion cells (RGC) that project to the rat pretectum, much of this evidence is circumstantial, and depends mostly upon electrophysiological data (e.g., conduction velocity). Here, we have used microinjections of Fluoro-Gold into the OPN (pretectum and superior colliculus as controls) to retrogradely label RGCs projecting to this region. The retinae were whole-mounted, viewed under fluorescence, and the regional distribution pattern, laterality of projection, and cell soma sizes determined. The results show OPN injections label a small subpopulation of RGCs. In the contralateral retinae, labeled RGCs were most numerous and widespread, with 97% projecting to the contralateral pretectum. The highest density of cells in the contralateral retinae was found in the inferior and nasal retinal quadrants. In the ipsilateral retinae, the small number of labeled cells were concentrated in the periphery of the inferior and nasal retinal quadrants. A striking feature of both ipsilateral and contralateral retinae was the paucity of labeled cells found in the dorsal hemiretina (lower visual field). Cell size measurements indicate 90-95% of labeled RGCs had diameters of 9-13 microm, while most of the remaining cells had diameters of 20-25 microm. This would suggest class III cells may be the predominant RGC type mediating pupilloconstriction, although a smaller population of larger cells (e.g., class I and/or II) may also contribute to this pathway. The recent reports utilizing the PLR as an assay for the efficacy of intraretinal grafts has highlighted the significance of the regional distribution results. The extremely low number of labeled cells in the dorsal hemiretina would argue for the placement of such grafts in the ventral hemiretina.