Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A millipore--blue dextran affinity binding system

R P Isseroff, G W Dietz

    Journal of Biochemical and Biophysical Methods
    |May 1, 1979
    PubMed
    Summary

    Blue dextran-adsorbed filter discs effectively bind lactic dehydrogenase, allowing specific elution with NADH. Alkaline phosphatase, however, did not bind, demonstrating selective enzyme capture and recovery.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Association of dopamine D2 receptor gene haplotypes with anovulation and fecundity in female Hispanics.

    Human reproduction (Oxford, England)·1994
    Same author

    Human tryptophan oxygenase localized to 4q31: possible implications for alcoholism and other behavioral disorders.

    Genomics·1991
    Same author

    Segond tibial condyle fracture: lateral capsular ligament avulsion.

    Radiology·1986
    Same author

    A new synaptosomal biosynthetic pathway of glutamate and GABA from ornithine and its negative feedback inhibition by GABA.

    Journal of neurochemistry·1982
    Same author

    Initial roentgenographic manifestations of pulmonary Mycobacterium tuberculosis, M kansasii, and M intracellularis infections.

    Chest·1981
    Same author

    Purification and peptide mapping of rat brain choline acetyltransferase.

    The Journal of biological chemistry·1980

    Area of Science:

    • Biochemistry
    • Enzyme immobilization
    • Affinity chromatography

    Background:

    • Enzyme purification and immobilization are crucial for biochemical applications.
    • Developing stable and selective supports for enzyme capture is an ongoing challenge.
    • Blue dextran is a known ligand with affinity for certain proteins.

    Purpose of the Study:

    • To develop a stable adsorbent for lactic dehydrogenase (LDH) using blue dextran.
    • To investigate the specificity of blue dextran-adsorbed filter discs for enzyme binding and elution.
    • To assess the utility of this method for enzyme purification.

    Main Methods:

    • Adsorption of blue dextran onto millipore filter discs.
    • Incubation of discs with lactic dehydrogenase and alkaline phosphatase.
    • Specific elution of bound enzymes using substrate (NADH).
    • Analysis of filtrate for enzyme recovery.

    Main Results:

    • Blue dextran-adsorbed discs demonstrated stable binding of lactic dehydrogenase.
    • Lactic dehydrogenase was specifically eluted using NADH.
    • Alkaline phosphatase showed no significant binding to the discs.
    • Complete recovery of alkaline phosphatase in the filtrate confirmed selectivity.

    Conclusions:

    • Millipore filter discs modified with blue dextran provide a stable and selective matrix for lactic dehydrogenase capture.
    • The method allows for specific elution of the target enzyme using its substrate, NADH.
    • This technique offers a promising approach for enzyme purification and immobilization.

    Related Experiment Videos