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Behavioral sensitization to amphetamine is not accompanied by a decrease in the number of c-Fos containing cells in

S M Turgeon1, J E Blum, E A Auerbach

  • 1Department of Psychology and Neuroscience Program, Amherst College, MA 01002, USA. smturgeon@amherst.edu

Brain Research
|March 31, 1998
PubMed
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Behavioral sensitization to amphetamine in rats involves changes in Fos-related antigen-like immunoreactivity (FRALI) but not c-Fos-like immunoreactivity (FLI) in the striatum. This suggests distinct cellular responses during amphetamine sensitization.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Pharmacology

Background:

  • Amphetamine-induced behavioral sensitization is a key model for studying drug addiction.
  • The expression of immediate early genes, such as c-Fos, is often used as a marker for neuronal activation.

Purpose of the Study:

  • To investigate the expression patterns of c-Fos-like immunoreactivity (FLI) and chronic Fos-related antigen-like immunoreactivity (FRALI) in the rat striatum during amphetamine sensitization.
  • To differentiate between FLI and FRALI responses to chronic amphetamine exposure.

Main Methods:

  • Male rats were treated with amphetamine (5 mg/kg) or vehicle for four consecutive days.
  • On the fifth day, animals received a challenge dose of amphetamine or vehicle.
  • Immunohistochemistry was used to quantify FLI- and FRALI-positive cells in the striatum.

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Main Results:

  • The number of FLI-positive cells in the striatum was increased in the V-A and A-A groups compared to the V-V control group.
  • The number of FRALI-positive cells in the striatum was increased in the A-V and A-A groups compared to the V-V control group.
  • These findings indicate that FLI and FRALI exhibit differential expression patterns following amphetamine treatment and challenge.

Conclusions:

  • The observed FLI and FRALI expression patterns suggest distinct roles in the neurobiological adaptations underlying amphetamine sensitization.
  • The results support the distinction between FLI and FRALI, ruling out antibody cross-reactivity as an explanation for FLI expression changes.
  • Behavioral sensitization to amphetamine is not associated with an altered number of striatal cells expressing c-Fos.