Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Type II DNA topoisomerases

J M Berger1

  • 1Whitehead Institute, Cambridge, MA 02142, USA. berger@wi.mit.edu

Current Opinion in Structural Biology
|March 31, 1998
PubMed
Summary
This summary is machine-generated.

Type II DNA topoisomerases are essential enzymes that transport DNA strands. Structural and biochemical studies reveal how these enzymes bind DNA and nucleotides, involving significant domain movements.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Trastuzumab in Addition to Chemotherapy in Patients With Metastatic Human Epidermal Growth Factor Receptor 2-Positive Gastroesophageal Cancer-A Real-World Experience.

Clinical oncology (Royal College of Radiologists (Great Britain))·2026
Same author

Peripheral blood DNA methylation profile and immune checkpoint inhibitor response in cancer patients.

ESMO open·2026
Same author

Predictive potential of patient-specific immunological characteristics in solid cancers: circulating monocytes, myeloid-derived suppressor cells, T cells, and the T-cell receptor repertoire.

ESMO open·2026
Same author

Prognosticators of survival in patients with metastatic pancreatic cancer and ascites.

ESMO open·2023
Same author

Clinical risk factors for ascites in metastatic pancreatic cancer.

ESMO open·2023
Same author

Precursor-product relationships of Poly(γ-glutamylcysteinyl)glycine biosynthesis in Datura innoxia.

Plant cell reports·2013
Same journal

Metabolic disruptions through a three-dimensional genomic lens.

Current opinion in structural biology·2026
Same journal

Collective variable design for biomolecular conformational dynamics.

Current opinion in structural biology·2026
Same journal

Polymer scaling in protein crowding: From dilute coils to semidilute meshes.

Current opinion in structural biology·2026
Same journal

Tuning the physicochemical properties of rationally designed protein-based biomolecular condensates.

Current opinion in structural biology·2026
Same journal

Editorial overview: Folding, binding and protein design.

Current opinion in structural biology·2026
Same journal

Macromolecular crowding reshapes the conformational landscapes of intrinsically disordered proteins: mechanisms, cellular contexts, and functional consequences.

Current opinion in structural biology·2026
See all related articles

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Structural Biology

Background:

  • Type II DNA topoisomerases are crucial enzymes involved in managing DNA topology.
  • These enzymes facilitate the passage of one DNA duplex through another, a vital process for DNA replication and transcription.

Purpose of the Study:

  • To elucidate the mechanistic details of the DNA transport reaction catalyzed by Type II DNA topoisomerases.
  • To identify the specific sites of DNA and nucleotide binding within the enzyme complex.

Main Methods:

  • Utilized a combination of structural analyses, likely including X-ray crystallography or cryo-electron microscopy.
  • Employed biochemical assays to investigate enzyme activity and binding interactions.

Main Results:

Related Experiment Videos

  • Revealed the precise locations of DNA binding within the enzyme.
  • Identified the nucleotide binding sites crucial for enzyme function.
  • Demonstrated the occurrence of large-scale domain motions during the DNA transport reaction.

Conclusions:

  • The study provides a detailed mechanistic understanding of Type II DNA topoisomerase function.
  • The findings highlight the importance of domain dynamics in the enzyme's catalytic cycle.