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Related Experiment Videos

Keratocyte repopulation in epikeratoplasty specimens

Y D Yoon1, G O Waring, R D Stulting

  • 1Department of Ophthalmology, Emory University School of Medicine, Atlanta, Georgia, USA.

Cornea
|March 31, 1998
PubMed
Summary

Keratocyte density in epikeratoplasty lenticules returns to normal levels around 48 months after surgery. This study observed keratocyte repopulation in human epikeratoplasty lenticules, showing migration from the periphery to the center.

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Area of Science:

  • Ophthalmology
  • Corneal Surgery
  • Histology

Background:

  • Epikeratoplasty involves the use of lenticules to reshape the cornea.
  • Understanding keratocyte repopulation is crucial for evaluating the long-term success of corneal grafts.

Purpose of the Study:

  • To investigate the histological characteristics of surgically removed human epikeratoplasty lenticules.
  • To analyze the pattern and timeline of keratocyte repopulation within these lenticules.

Main Methods:

  • Histological and ultrastructural examination of 15 epikeratoplasty lenticules.
  • Quantification of keratocyte density per high-power field.
  • Comparison of keratocyte density with age-matched controls.

Main Results:

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  • Keratocyte density in lenticules increased over time, reaching levels similar to controls (30-40 cells/HPF) by approximately 48 months.
  • Keratocytes appeared to migrate from the periphery towards the central region of the lenticules.
  • Indications for surgery included myopia, keratoconus, and aphakia.

Conclusions:

  • Normal keratocyte density is achieved in epikeratoplasty lenticules approximately 48 months post-surgery.
  • The findings suggest a gradual repopulation and normalization process for corneal cells after epikeratoplasty.