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Antisense oligonucleotides
N Kashihara1, Y Maeshima, H Makino
1Department of Medicine III, Okayama University Medical School, Japan. kashinao@med.okayama-u.ac.jp
Experimental Nephrology
|April 2, 1998
Abstract:
Antisense technology was developed to inhibit gene expression by utilizing an oligonucleotide complementary to the mRNA which encodes the target gene. There are a few possible mechanisms for the inhibitory effects of antisense oligonucleotides. Among them, degradation of mRNA by RNase H is considered to be the major mechanism of action for antisense oligonucleotides. This technique was originally used to elucidate the function of a target gene, but may also have therapeutic applications, provided it is designed carefully and properly.