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Stealth PLA-PEG nanoparticles as protein carriers for nasal administration
1Dept. de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia, Santiago de Compostela, Spain.
Pharmaceutical Research
|April 2, 1998
Summary
Surface-modified nanoparticles effectively deliver proteins via the nasal route. PLA-PEG nanoparticles enhanced tetanus toxoid transport across the nasal mucosa into circulation and lymph nodes.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Drug Delivery
Background:
- Protein antigen delivery presents challenges for effective mucosal immunization.
- Nanoparticles offer a promising platform for enhanced protein transport and stability.
Purpose of the Study:
- To encapsulate tetanus toxoid (TT) in hydrophobic (PLA) and surface- hydrophilic (PLA-PEG) nanoparticles.
- To evaluate the efficacy of these nanoparticles for protein transport across the nasal mucosa.
Main Methods:
- Nanoparticles prepared using a modified solvent evaporation technique.
- Characterization of size, zeta potential, and hydrophobicity.
- In vitro assessment of active antigen delivery and in vivo intranasal administration in rats with radiolabeled TT.
Main Results:
- PLA and PLA-PEG nanoparticles exhibited similar sizes (137-156 nm) and negative charges but differed in hydrophobicity.
- PLA-PEG nanoparticles, particularly with gelatin, ensured extended active protein delivery.
- PLA-PEG nanoparticles significantly enhanced TT transport into blood and lymph nodes compared to PLA nanoparticles.
- High radioactivity levels persisted in blood for 48 hours post-administration of 125I-TT-loaded PLA-PEG nanoparticles.
Conclusions:
- A novel nanoparticulate system was developed for effective protein transport via the nasal mucosa.
- Surface properties of nanoparticles critically influence protein transport across the nasal barrier.