Susceptibility of different isolates of Vibrio harveyi to antibiotics

Area of Science:

• Microbiology
• Aquatic Animal Health
• Antimicrobial Resistance

Background:

• Vibrio harveyi is a significant pathogen affecting penaeid shrimp and other marine organisms.
• Understanding the antimicrobial susceptibility patterns of V. harveyi is crucial for disease management in aquaculture.
• Antibiotic resistance in V. harveyi can complicate treatment strategies and impact stock health.

Purpose of the Study:

• To determine the antibiotic susceptibility profiles of Vibrio harveyi strains.
• To compare the resistance patterns of V. harveyi strains isolated from different geographical locations and sources.
• To identify potential resistance markers in specific V. harveyi populations.

Main Methods:

• Isolation and identification of six Vibrio harveyi strains from diseased penaeids, seawater, and diseased Talorchestia sp.
• Antimicrobial susceptibility testing using standard antibiotic discs or agar dilution methods.
• Analysis of resistance patterns against a panel of commonly used antibiotics.

Main Results:

• Three Vibrio harveyi strains isolated in Taiwan demonstrated resistance to nitrofurantoin, novobiocin, and sulphonamide.
• Two reference strains (ATCC 25919, ATCC 14126) and one strain from Indonesia were susceptible to these three antibiotics.
• Varied susceptibility patterns were observed among the tested V. harveyi strains, indicating geographical or host-specific differences.

Conclusions:

• Geographical origin significantly influences the antibiotic resistance profiles of Vibrio harveyi.
• Vibrio harveyi strains from Taiwan exhibit notable resistance to certain commonly used antibiotics.
• Further research is needed to monitor and manage antibiotic resistance in V. harveyi to ensure effective disease control in aquaculture.