Social stress in loser rats: opposite immunological effects in submissive and subdominant males

The impact of chronic confrontation on behavior, body weight, and aspects of blood cellular immunity was investigated in 24 Long Evans intruder rats. At the beginning of a 1-week confrontation period, an adult male was introduced into a one male-one female resident group which resulted in fights for dominance. Although most intruders became losers in this situation, their specific behavior differed. Two groups of losers were defined: subdominant (N = 11) and submissive intruders (N = 9). In contrast to subdominant intruders, submissive males often displayed behaviors indicating social defeat. They were frequently involved in agonistic interactions and lost 7% of their initial body weight. Confrontation provoked marked changes in many measures of blood cellular immunity. Importantly, the immunological effects differed significantly in magnitude and direction depending on the individuals' behavior. Submissive males reacted with reduction in lymphocyte proliferation in response to ConA and shifts in lymphocyte subsets (decline in percentage of CD4 and CD8 T cells, increase of B cells), but with little change in lymphocyte numbers. In addition, a pronounced increase in granulocytes was evident. In contrast, in subdominant males lymphocyte, T cell, and B cell counts were lowered but no change in subset composition occurred. The effects on T and B cell composition were not fully reversible within 7 days after end of the confrontation period indicating long-term consequences for migration of lymphocyte subsets. These data show that a detailed behavioral analysis is required for a meaningful biological evaluation of social stress-induced immune changes in rats, because different coping strategies result in different immunological consequences.