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Related Experiment Videos

Stored renin isoforms in the developing rat kidney

L L Norling1, T M Smith, J R Ingelfinger

  • 1Pediatric Nephrology Unit, Massachusetts General Hospital, Boston 02114, USA. norling@helix.MGH.Harvard.edu

American Journal of Hypertension
|April 2, 1998
PubMed
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Fetal and adult rat kidneys store different renin isoforms with distinct chemical and enzymatic properties. These renin differences may influence the developing intrarenal renin-angiotensin system regulation.

Area of Science:

  • Biochemistry
  • Physiology
  • Developmental Biology

Background:

  • Renin, a key enzyme in the renin-angiotensin system, is present in fetal and adult rat kidneys.
  • Renin localization differs, with fetal kidney renin in microvasculature and adult kidney renin in juxtaglomerular cells.
  • Potential chemical and functional differences in renin isoforms between fetal and adult kidneys warrant investigation.

Purpose of the Study:

  • To compare stored renin isoforms in fetal and adult rat kidneys.
  • To investigate potential chemical and functional distinctions in renin isoforms.
  • To explore the implications of these differences on intrarenal renin-angiotensin system regulation during development.

Main Methods:

  • Renin isolation from fetal and adult rat kidney homogenates using pepstatin agarose.

Related Experiment Videos

  • Separation of renin isoforms by molecular size (SDS-PAGE) and isoelectric point (2D gel electrophoresis).
  • Identification of isoforms via silver staining or immunoblotting; enzymatic activity measured by Angiotensin I generation.

    • Main Results:

    • A ~45 kDa renin band was identified in both fetal and adult kidneys.
    • Fetal kidney renin isoforms exhibited a more acidic isoelectric point (pI 5.3) compared to adult kidney renin (pI 6.0).
    • Fetal kidneys showed significantly higher inactive renin and total renin activity, but lower active renin compared to adult kidneys.

    Conclusions:

    • Significant differences exist in the physical and enzymatic characteristics of stored renin in fetal versus adult rat kidneys.
    • These variations in renin isoforms suggest a role in the developmental regulation of the intrarenal renin-angiotensin system.
    • The distinct renin profiles may contribute to differential control of blood pressure and fluid homeostasis during kidney development.