Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA extraction from human urinary sediment

M Yokota1, N Tatsumi, I Tsuda

  • 1Department of Clinical and Laboratory Medicine, Osaka City University Medical School, Japan.

Journal of Clinical Laboratory Analysis
|April 3, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Increased Survival Benefit of Adjuvant Intra-arterial Infusion Chemotherapy in HCC Patients with Portal Vein Infiltration after Hepatectomy.

World journal of surgery·2020
Same author

Incidence of and risk factors for lymphocele formation after lateral pelvic lymph node dissection for rectal cancer: a retrospective study.

Colorectal disease : the official journal of the Association of Coloproctology of Great Britain and Ireland·2019
Same author

3D printed model-based simulation of laparoscopic surgery for descending colon cancer with a concomitant abdominal aortic aneurysm.

Techniques in coloproctology·2019
Same author

Obstruction is associated with perineural invasion in T3/T4 colon cancer.

Colorectal disease : the official journal of the Association of Coloproctology of Great Britain and Ireland·2019
Same author

Influenza A virus infection of vascular endothelial cells induces GSK-3β-mediated β-catenin degradation in adherens junctions, with a resultant increase in membrane permeability.

Archives of virology·2014
Same author

Abundant daily non-sedentary activity is associated with reduced prevalence of metabolic syndrome and insulin resistance.

Journal of endocrinological investigation·2013
Same journal

IGFBP3 As a Potential Biomarker and Therapeutic Target in Hepatocellular Carcinoma: A Multi-Cohort Analysis.

Journal of clinical laboratory analysis·2026
Same journal

Optimization of Reflex Testing Rules on the Sysmex XN-9100 Automated Hematology Line Improves Efficiency and Reduces Repeat Testing in Different Work Areas.

Journal of clinical laboratory analysis·2026
Same journal

sFlt-1/PlGF Ratio in the Diagnosis of Preeclampsia on the MAGLUMI X3 Analyzer.

Journal of clinical laboratory analysis·2026
Same journal

Application of Cell-Free DNA Barcode-Enabled Single-Molecule Test for Non-Invasive Prenatal Testing of α-Thalassemia and β-Thalassemia.

Journal of clinical laboratory analysis·2026
Same journal

Changes of CEA Diagnostic Performance Under the New Reference Interval Stratified by Age and Sex.

Journal of clinical laboratory analysis·2026
Same journal

Investigation of Specific IgG-Secreting Cells in Congenital Toxoplasmosis: The TOXODIAG Study.

Journal of clinical laboratory analysis·2026
See all related articles

Urine DNA extraction is effective for genetic analysis, even with microbial DNA present. Stored urine samples yield stable DNA for identification and sex determination via polymerase chain reaction (PCR).

Area of Science:

  • Forensic Science
  • Molecular Biology
  • Genetics

Background:

  • Urine is a readily available biological sample.
  • DNA extraction from urine presents challenges due to low concentrations and potential inhibitors.
  • Previous methods for urine DNA analysis were limited.

Purpose of the Study:

  • To assess the feasibility of DNA extraction from human urine sediments.
  • To determine the stability of urine DNA for downstream genetic analyses.
  • To evaluate urine as a source for polymerase chain reaction (PCR)-based forensic and genetic studies.

Main Methods:

  • DNA extraction from fresh and ethanol-fixed urinary sediments.
  • Quantification of extracted DNA yield.
  • Polymerase chain reaction (PCR) for DNA amplification and analysis.

Related Experiment Videos

  • Enzymatic analysis of extracted DNA.

    • Main Results:

    • Sufficient DNA was extracted from urinary sediments for PCR and enzymatic analysis.
    • DNA yield ranged from trace amounts to 20 micrograms per 10 mL of urine.
    • DNA remained stable in ethanol-fixed samples for at least 2 weeks.
    • Individual identification and sex determination were successful using both fresh and stored urine samples.

    Conclusions:

    • Urine is a viable and stable source for DNA extraction.
    • DNA from urine sediments can be reliably used for PCR-based investigations.
    • Genetic studies and forensic applications can utilize urine samples effectively.