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Related Experiment Videos

The telomere and telomerase: how do they interact?

E Blackburn1, A Bhattacharyya, D Gilley

  • 1Department of Microbiology and Immunology, University of California, San Francisco 94143-0414, USA.

Ciba Foundation Symposium
|January 1, 1997
PubMed
Summary
This summary is machine-generated.

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Telomere length is regulated by telomerase, an enzyme that copies RNA to DNA. Mutations in telomerase RNA or telomere-binding proteins disrupt this process, affecting DNA replication and cell division.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Telomeres, the ends of eukaryotic chromosomes, are maintained by the enzyme telomerase.
  • Telomerase synthesizes telomeric DNA repeats using an RNA template.
  • A telomere homeostasis system regulates telomere length, involving telomeres and telomerase.

Purpose of the Study:

  • To investigate the roles of telomere and telomerase in maintaining telomere length homeostasis.
  • To analyze the effects of mutations in telomerase RNA template sequences on telomere maintenance and cell division.

Main Methods:

  • Site-directed mutagenesis of telomerase RNA template sequences in Tetrahymena and yeast (Kluyveromyces lactis).
  • Analysis of telomere length, DNA-protein complex structure, and Rap1p binding.

Related Experiment Videos

  • Investigation of cell cycle progression, specifically anaphase block, in response to telomere alterations.

    • Main Results:

    • Mutations in telomerase RNA template residues affected critical telomerase functions.
    • Altered telomeric sequences induced anaphase block in Tetrahymena micronuclei.
    • Template mutations in K. lactis telomerase RNA led to unregulated telomere elongation, linked to Rap1p binding loss.

    Conclusions:

    • Telomere structure and DNA length are critical for regulating telomerase access and action.
    • Telomere length homeostasis is a complex system influenced by telomerase activity and DNA-protein complex formation.