Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The telomere and telomerase: how do they interact?

E Blackburn1, A Bhattacharyya, D Gilley

  • 1Department of Microbiology and Immunology, University of California, San Francisco 94143-0414, USA.

Ciba Foundation Symposium
|January 1, 1997
PubMed
Summary

Telomere length is regulated by telomerase, an enzyme that copies RNA to DNA. Mutations in telomerase RNA or telomere-binding proteins disrupt this process, affecting DNA replication and cell division.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Adolescents and young adults with TB in a low-incidence setting.

IJTLD open·2025
Same author

Two Characteristic Contributions to the Superconducting State of 2H-NbSe_{2}.

Physical review letters·2025
Same author

Sweet liking predicts liking and familiarity of some alcoholic beverages, but not alcohol intake: A population study using a split-sample approach.

Food research international (Ottawa, Ont.)·2024
Same author

Ligand structure and diluent nature in defining improved Am<sup>3+</sup> and Cm<sup>3+</sup> separation using diglycolamides: a combined solvent extraction and DFT study.

Dalton transactions (Cambridge, England : 2003)·2024
Same author

Health care professionals' experiences of pain management in the intensive care unit: a qualitative study.

Anaesthesia·2023
Same author

Responsiveness to warning sensations and anxiety-related psychological traits modulate individual differences in preference for vegetable foods with varied sensory properties.

Food research international (Ottawa, Ont.)·2023

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Telomeres, the ends of eukaryotic chromosomes, are maintained by the enzyme telomerase.
  • Telomerase synthesizes telomeric DNA repeats using an RNA template.
  • A telomere homeostasis system regulates telomere length, involving telomeres and telomerase.

Purpose of the Study:

  • To investigate the roles of telomere and telomerase in maintaining telomere length homeostasis.
  • To analyze the effects of mutations in telomerase RNA template sequences on telomere maintenance and cell division.

Main Methods:

  • Site-directed mutagenesis of telomerase RNA template sequences in Tetrahymena and yeast (Kluyveromyces lactis).
  • Analysis of telomere length, DNA-protein complex structure, and Rap1p binding.

Related Experiment Videos

  • Investigation of cell cycle progression, specifically anaphase block, in response to telomere alterations.

    • Main Results:

    • Mutations in telomerase RNA template residues affected critical telomerase functions.
    • Altered telomeric sequences induced anaphase block in Tetrahymena micronuclei.
    • Template mutations in K. lactis telomerase RNA led to unregulated telomere elongation, linked to Rap1p binding loss.

    Conclusions:

    • Telomere structure and DNA length are critical for regulating telomerase access and action.
    • Telomere length homeostasis is a complex system influenced by telomerase activity and DNA-protein complex formation.