Jove
Visualize
Contact Us

Related Experiment Videos

Telomeres in the haemopoietic system

P M Lansdorp1, S Poon, E Chavez

  • 1Terry Fox Laboratory, British Columbia Cancer Research Centre, Vancouver, Canada.

Ciba Foundation Symposium
|January 1, 1997
PubMed
Summary

Hematopoietic stem cells have limited divisions, evidenced by telomere shortening. A new technique quantifies telomere repeats to study their role in blood cell production and cancer.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Search for Light Pseudoscalar Bosons, Pair-Produced in Higgs Boson Decays in the Four-Electron Final State in Proton-Proton Collisions at sqrt[s]=13  TeV.

Physical review letters·2026
Same author

First Evidence for Mixing-Induced CP Violation in B_{s}^{0}→J/ψϕ(1020) Decays in pp Collisions at sqrt[s]=13  TeV.

Physical review letters·2026
Same author

Observation of Suppressed Charged-Particle Production in Ultrarelativistic Oxygen-Oxygen Collisions.

Physical review letters·2026
Same author

Measurement of D^{0} Meson Photoproduction in Ultraperipheral Heavy Ion Collisions.

Physical review letters·2026
Same author

Observation of tWZ Production at the CMS Experiment.

Physical review letters·2026
Same author

First Exclusive Reconstruction of the B^{*+}, B^{*0}, and B_{s}^{*0} Mesons and Precise Measurement of Their Masses.

Physical review letters·2026
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Area of Science:

  • Hematology
  • Stem Cell Biology
  • Genetics

Background:

  • Continuous blood cell production is vital, requiring over 10^12 cells daily in adults.
  • Adult bone marrow harbors mostly quiescent hematopoietic stem cells (HSCs).
  • HSCs possess self-renewal and differentiation potential, generating all blood cell types.

Purpose of the Study:

  • To investigate the role of telomeres in normal and malignant hematopoiesis.
  • To explore the hypothesis that HSCs have a limited replicative potential.
  • To introduce a novel method for telomere length assessment.

Main Methods:

  • Development of a quantitative fluorescence in situ hybridization (qFISH) technique.
  • Assessment of telomere repeat number at individual chromosome ends.

Related Experiment Videos

  • Analysis of telomere length changes in purified fetal and adult stem cell fractions.

    • Main Results:

    • Purified candidate stem cells exhibited functional differences in turnover and self-renewal capacity.
    • Functional differences correlated with measurable telomere repeat loss.
    • Low levels of telomerase were detected in all purified cell fractions, despite telomere shortening.

    Conclusions:

    • Telomere attrition may limit the replicative lifespan of hematopoietic stem cells.
    • The qFISH technique is a valuable tool for studying telomere dynamics in hematopoiesis.
    • Understanding telomere function is crucial for both normal blood formation and cancer research.