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Adenovirus internalization and infection require dynamin

K Wang1, S Huang, A Kapoor-Munshi

  • 1Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA.

Journal of Virology
|April 3, 1998
PubMed
Summary

Adenovirus uses clathrin-mediated endocytosis for cell entry. Researchers found that dynamin, a protein regulating this process, is crucial for adenovirus internalization and infection in HeLa cells.

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Area of Science:

  • Virology
  • Cell Biology
  • Molecular Biology

Background:

  • Cell receptors for adenovirus internalization are known.
  • The specific pathway for adenovirus entry and infection remains unclear.

Purpose of the Study:

  • To investigate the mechanism of adenovirus entry into cells.
  • To determine the role of dynamin in adenovirus infection.

Main Methods:

  • Adenovirus entry and infection were studied in HeLa cells.
  • Dynamin function was manipulated using mutant dynamin expression (lacking or overexpressing).
  • Clathrin-mediated endocytosis was assessed.

Main Results:

  • Mutant dynamin expression significantly reduced adenovirus internalization.

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  • Gene delivery by adenovirus was also significantly decreased.
  • These results suggest dynamin's essential role in the process.

    • Conclusions:

    • Adenovirus entry into HeLa cells functionally requires dynamin.
    • The findings support a model where adenovirus enters cells via clathrin-coated pits.