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Adenovirus internalization and infection require dynamin
K Wang1, S Huang, A Kapoor-Munshi
1Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA.
Abstract:
The cell receptors that facilitate adenovirus internalization into cells have been identified; however, the infectious pathway of virus entry has not been established. Adenovirus entry and infection were examined in HeLa cells lacking or overexpressing mutant dynamin, a protein that specifically regulates clathrin-mediated endocytosis. Expression of mutant dynamin significantly reduced adenovirus internalization and gene delivery, indicating a functional requirement for this molecule. These findings are consistent with virus entry via the clathrin-coated pit pathway.
Insights
Adenovirus uses clathrin-mediated endocytosis for cell entry. Researchers found that dynamin, a protein regulating this process, is crucial for adenovirus internalization and infection in HeLa cells.
Area of Science:
- Virology
- Cell Biology
- Molecular Biology
Background:
- Cell receptors for adenovirus internalization are known.
- The specific pathway for adenovirus entry and infection remains unclear.
Purpose of the Study:
- To investigate the mechanism of adenovirus entry into cells.
- To determine the role of dynamin in adenovirus infection.
Main Methods:
- Adenovirus entry and infection were studied in HeLa cells.
- Dynamin function was manipulated using mutant dynamin expression (lacking or overexpressing).
- Clathrin-mediated endocytosis was assessed.
Main Results:
- Mutant dynamin expression significantly reduced adenovirus internalization.
- Gene delivery by adenovirus was also significantly decreased.
- These results suggest dynamin's essential role in the process.
Conclusions:
- Adenovirus entry into HeLa cells functionally requires dynamin.
- The findings support a model where adenovirus enters cells via clathrin-coated pits.