Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Vaginal immunization with recombinant gram-positive bacteria

D Medaglini1, M R Oggioni, G Pozzi

  • 1Dipartimento di Biologia Molecolare, Università di Siena, Italy.

American Journal of Reproductive Immunology (New York, N.Y. : 1989)
|April 4, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spontaneous complexity in the dynamics of slow laboratory earthquakes.

Nature communications·2025
Same author

Geometric control by active mechanics of epithelial gap closure.

Soft matter·2024
Same author

T cell therapy against cancer: A predictive diffuse-interface mathematical model informed by pre-clinical studies.

Journal of theoretical biology·2022
Same author

The Föppl-von Kármán equations of elastic plates with initial stress.

Royal Society open science·2022
Same author

Author Correction: Phase variation in pneumococcal populations during carriage in the human nasopharynx.

Scientific reports·2020
Same author

Phase variation in pneumococcal populations during carriage in the human nasopharynx.

Scientific reports·2020

This study shows that using live, nonpathogenic Gram-positive bacteria as vaccine vectors can effectively induce immune responses in the genital mucosa. This approach shows promise for developing vaccines against sexually transmitted diseases (STDs).

Area of Science:

  • Microbiology
  • Immunology
  • Vaccinology

Background:

  • Genital mucosa is a primary entry point for various viral and bacterial pathogens.
  • Developing vaccines against sexually transmitted diseases (STDs) requires inducing a protective immune response at the genital mucosa.

Purpose of the Study:

  • To evaluate the potential of nonpathogenic Gram-positive bacteria as live vaccine vectors for STDs.
  • To assess the ability of these vectors to induce mucosal and systemic immunity.

Main Methods:

  • Constructed recombinant Gram-positive bacteria (Streptococcus gordonii, Lactobacillus spp.) expressing heterologous antigens.
  • Administered bacteria vaginally to Balb/c mice and Cynomolgus monkeys.
  • Monitored bacterial colonization and measured local (vaginal IgA) and systemic (serum IgG) immune responses.

Related Experiment Videos

Main Results:

  • Streptococcus gordonii and Lactobacillus casei stably colonized the murine vagina after single inoculation.
  • Recombinant S. gordonii expressing human papillomavirus (HPV) and human immunodeficiency virus (HIV) antigens induced antigen-specific vaginal IgA and serum IgG in mice.
  • Intravaginal immunization with recombinant S. gordonii elicited local and systemic immune responses in monkeys.

Conclusions:

  • Nonpathogenic Gram-positive bacteria can serve as effective live vaccine vectors for mucosal immunization.
  • This strategy holds significant potential for the development of novel vaccines against STDs.