Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Anomalous azide binding to metmanganomyoglobin

B M Hoffman, Q H Gibson

    Biochemistry
    |August 10, 1976
    PubMed
    Summary
    This summary is machine-generated.

    Metmanganomyoglobin (MnIIIMb) reacts with azide, showing kinetic complexity. Spectrophotometry reveals an equilibrium between two azide complexes, suggesting an intermediate species.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Postoperative cognitive dysfunction and mortality following lung transplantation.

    American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2017
    Same author

    Psychosocial Predictors of Mortality Following Lung Transplantation.

    American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2015
    Same author

    Changes in neurocognitive functioning following lung transplantation.

    American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2012
    Same author

    The ratios of iron to oxygen, iron to colour and oxygen to colour in the blood of men and women.

    The Journal of physiology·2010
    Same author

    Familial idiopathic methaemoglobinaemia and its treatment with ascorbic acid.

    Clinical science·2010
    Same author

    Cardiovascular disease risk, vascular health and erectile dysfunction among middle-aged, clinically depressed men.

    International journal of impotence research·2009
    Same journal

    Aromatic Cage-Directed Azide-Methyllysine Photochemistry for Profiling Nonhistone Interacting Partners of the MeCP2 Methyl-CpG-Binding Domain.

    Biochemistry·2026
    Same journal

    Differential Hydroxypyruvate Processing by <i>E. coli</i> and <i>P. aeruginosa</i> DXP Synthases Reveals Preferential Xylulose 5-Phosphate Formation by the <i>P. aeruginosa</i> Enzyme.

    Biochemistry·2026
    Same journal

    Structural and Functional Characterization of Heterologous Nitrogenase Complexes.

    Biochemistry·2026
    Same journal

    Discovery of Bacterial Unspecific Peroxygenases.

    Biochemistry·2026
    Same journal

    Lactate Biology: Subcellular Routing and Chemical Form Define Function.

    Biochemistry·2026
    Same journal

    Nature's Anaerobic Toolkit: Glycyl Radical Enzymes and Their Expanding Functional and Mechanistic Diversity.

    Biochemistry·2026
    See all related articles

    Area of Science:

    • Biochemistry
    • Chemical Kinetics
    • Spectroscopy

    Background:

    • Metmanganomyoglobin (MnIIIMb) is a biologically relevant metalloprotein.
    • Azide is a known ligand that interacts with metalloproteins.
    • Understanding ligand binding kinetics is crucial for protein function studies.

    Purpose of the Study:

    • To investigate the reaction kinetics between metmanganomyoglobin and azide.
    • To characterize the nature of the azide complex formed with MnIIIMb.
    • To elucidate the kinetic complexity observed in this reaction.

    Main Methods:

    • Spectrophotometric analysis of the reaction between MnIIIMb and azide.
    • Kinetic analysis to determine reaction patterns.
    • Low-temperature studies (3 degrees C) to observe spectral changes.

    Related Experiment Videos

    Main Results:

    • The reaction of MnIIIMb with azide exhibits novel kinetic complexity.
    • Spectrophotometric data indicate that the azide complex does not fully form, even at high azide concentrations.
    • An equilibrium exists between a final azide complex and an intermediate species spectrally similar to MnIIIMb.

    Conclusions:

    • The kinetic complexity arises from an equilibrium between two spectroscopically distinct azide complexes.
    • An intermediate, weak Mn3+-azide complex is proposed, with the metal ion out-of-plane.
    • A "final" complex is formed where the metal ion shifts towards the azide anion.